Proliferating Normal Human Tracheal Epithelial Cells-P0 (NhTE-P0)
$595.00
Proliferating Normal Human Tracheal Epithelial Cells- P0 (NhTE-P0) are isolated from the trachea of healthy human donors but do not contain epithelial cells from the primary bronchus. Each vial of the Proliferating NhTE-P0 cells contains the complete tracheal cell composition consisting of differentiated cells like ciliated and mucin producing cells as well as basal progenitor cells.
Proliferating NhTE-P0 cells have been characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5).
The NhTE-P0 cells are guaranteed to have minimum of at least 30 population doublings from passage number zero (P0) following the instructions and conditions provided by Novabiosis.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.
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Cryopreserved Human Tracheal Epithelial Cells P-0 Product Literature
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Product Storage
Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage tank. Cells are viable for at least 2 years when stored under these conditions.
Quality Control Testing
QC testing results provided will consist of the following:
Date of manufacture: MM/DD/YYYY
Attachment from P0 thaw: Typically 15-35% of initial count
Number of population doublings: Typically 25 (P1 to P3 in BEGM)
Average population doubling time: Typically 40 ± 10 hours (P1 to P2 cells)
Transepithelial resistance at 21 days ALI: Ttypically range from 500 to 2000 Ω (P0 cells starting material, 12mm Transwell™ format)
Percent ciliated cells at 21 days ALI: Typically >30% of P0 cells
Percent ciliated cells at 28 days ALI: Typically >40% of P0 cells
Immunofluorescence at Day 28:
P0 cells seeded at 350,000 cells/12mm Transwell™ and grown in ALI differentiation medium
Donor Characteristics Include: Donor age, sex, race, diabetic status, smoking history, alcohol use, and any co-morbidities
Serology results include (positive or negative): CMV, EBV HBV, HCV, HIV-1, HIV-2, and HLTV-1 and HLTV-2 (only if performed)
Culture results include (positive or negative): Gram +, Gram -, Mycoplasm and Fungi
Product Warranty
CULTURES HAVE A LIMITED LIFESPAN IN VITRO.
Novabiosis guarantees the performance of its cells only if following Novabiosis specific use instructions exclusively, and the recommended protocols are followed. The performance of the cells are not guaranteed if any modifications are made.
Cryopreserved proliferating NhTE-p0 are assured to be viable and functional when thawed and maintained properly.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.
WARNING: Primary human cell products are derived from whole human organs, thus should be treated as potentially infectious. Blood samples from the donor are tested and found non-reactive using FDA approved test kits in CLIA certified labs and are testing for the presence of HIV-I/II, Hepatitis B Virus and Hepatitis C Virus. In some cases, but not all, HTLV-1 and 2 are tested and reported.
Test results do not offer complete assurance that HIV-I/II, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be handled at the appropriate Biological Safety Level in order to minimize exposure of potentially infectious products.
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