Proliferating Normal Human Small Airway Epithelial Cells-P0 (NhSAE-P0)
$595.00
Proliferating Normal Human Small Airway Epithelial Cells- P0 (NhSAE-P0) are isolated from the fifth generation of bronchi down to small airways near 1mm in diameter. The cell composition consists of undifferentiated ciliated, non-ciliated, goblet, and basal cells.
Proliferating NhSAE-P0 cells have been characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), and tight junctions (Zonula Occludens-1).
The NhSAE-P0 cells are guaranteed to have minimum population doublings of at least 20 (from P0 to P2) following the instructions and conditions provided by Novabiosis.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.
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Cryopreserved Human Small Airway Epithelial Cells P-0 Product Literature
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Product Storage
Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage tank. Cells are viable for at least 2 years when stored under these conditions.
Quality Control Testing
QC testing results provided will consist of the following:
Date of Manufacture: MM/DD/YYY
Attachment from P0 thaw: Typically 20-30% of initial count
Number of population doublings: Typically 25 (P0 to P2 in BEGM)
Average population doubling time: Typically 40 ± 5 hours (P1 to P2 cells)
Transepithelial resistance at 21 days ALI: Typically range from 500 to 2000 Ω (P0 cells starting material, 12mm)
Percent ciliated cells at 21 days ALI: Typically >50% at P1 (P0 cells starting material)
Donor Characteristics Include: Donor age, sex, race, diabetic status, smoking history, alcohol use, and any co-morbidities
Serology results include (positive or negative): CMV, EBV HBV, HCV, HIV-1, HIV-2. Potentially HLTV-1 and HLTV-2 (if performed).
Culture results include (positive or negative): Gram +, Gram -, Mycoplasm and Fungi
Product Warranty
CULTURES HAVE A LIMITED LIFESPAN IN VITRO.
Novabiosis guarantees the performance of its cells only if following Novabiosis specific use instructions exclusively, and the recommended protocols are followed. The performance of the cells are not guaranteed if any modifications are made.
Cryopreserved proliferating NhSAE-p0 are assured to be viable and functional when thawed and maintained properly.
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THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.
WARNING: Primary human cell products are derived from whole human organs, thus should be treated as potentially infectious. Blood samples from the donor are tested and found non-reactive using FDA approved test kits in CLIA certified labs and are testing for the presence of HIV-I/II, Hepatitis B Virus and Hepatitis C Virus. In some cases, but not all, HTLV-1 and 2 are tested and reported.
Test results do not offer complete assurance that HIV-I/II, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be handled at the appropriate Biological Safety Level in order to minimize exposure of potentially infectious products.
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